ABSTRACT
Objective:To detect the levels of T help cell 17 (Th17)/regulatory T cell (Treg) in the peripheral blood of patients with ovarian cancer and explore their clinical significances.Methods:55 patients with ovarian cancer and 60 normal controls were enrolled.The percentage of Th17/Treg cells were detected by flow cytometry (FCM).The expression of retinoid-related orphan receptor gamma-t ( RORγt)/foxhead winged-helix box protein 3 ( Foxp3) mRNA was measured by real time PCR and the plasma levels of IL-17/TGF-β1 were detected by enzyme linked immunosorbent assay (ELISA).Results: The percentage of CD4+IL-17+/CD4+T cells,the mRNA expression of RORγt and the plasma levels of IL-17 increased significantly in patients with ovarian cancer than in normal controls (P<0.05).Compared with normal controls,the percentage of CD4+Foxp3+Treg/CD4+T cells,the mRNA expression of Foxp3 and the plasma levels of TGF-β1 increased significantly in patients with ovarian cancer ( P<0.05).Conclusion:The imbalance of Th17/Treg cells may exist in the peripheral blood of patients with ovarian cancer.Th17/Treg cells may participate the pathology of ovarian cancer.
ABSTRACT
Objective To study the clinical efficacy of human blood albumin and small dose heparin in treatment of severe preeclampsia and its effect on the expression of Endoglin,vascular endothelial growth factor (VEGF)and its receptors (Flt)in placental tissue.Methods 60 cases of severe preeclampsia collected in weifang yidu central hospital from October 2012 to January 2014 were randomly divided into experimental group and control group,each had 30 cases.Control group were given oral antihypertensive,expansion and diuretic treatment.Experiment group were received injection with 500 mL 5% glucose,60 mL 25% magnesium sulfate and 25 mg heparin on the basis of control group,one time each day,for successive seven days.Another 30 cases of normal full-term pregnancy for cesarean section delivery collected in our hospital during the same period were as blank group.Mean arterial pressure (Kpa)and 24 h urine protein quantitative and clinical efficacy were observed and compared between experimental group and control group before and after treatment.curative effect. Microscopically observed treatment after Endoglin in blank group, control group and experimental group placenta tissue,The expression of Endoglin,VEGF and Flt 1 in placental tissue among three groups were observed by microscope, and their positive expression rate were compared. Results The mean arterial pressure and urine protein were all improved after treatment in control group,and experimental group,but the latter was more obvious than the former(P<0.05 ).The expression location of Endoglin,VEGF and PLT-1 in three groups were the same with microscopic observation.Compared with blank group,the positive expression rate of Endoglin,VEGF protein and PLT-1 in placenta of other two groups were higher(P<0.05),and those in experimental group were more higher than control group(P<0.05).Conclusion Human blood albu min and small dose heparin can effectively improve the indexes of quantitative Kpa and 24 h urine protein in severe preeclampsia, reduce the expression of Endoglin,VEGF and Flt 1 protein in placental tissue.
ABSTRACT
Objective This study was aimed to determine the expression of matrix metalloproteinase-11 ( MMP-11 ) and MMP-12 in cervical cancer and the relationship between their expression with clinicopathological characteristics and the potential use as a prognostic and therapeutic agent.Methods Streptavidin-biotin-protein-linked peroxidase method(ie,SP) immunohistochemical method was used to detect the expression of MMP-11,MMP-12 in 20 cases of normal cervical tissue,36 cases of CIN tissue,g0 cases of cervical cancer.MMP-11 and 12 expression in cervical cancer and clinicopathological parameters of relevance were analyzed,and the relationship between MMP-1 1,-12 was studied in the development of cervical cancer for a preliminary study.Results The positive expression rate (82.50％,g1.25％ )of MMP-11 and-12 in cervical cancer was significantly higher than that of cervical dysplasia ( CIN ) specimens ( 69.44％,61.11％ ) and normal cervical tissues(5.00％,0),the difference was statistically significant ( P ＜0.05).(2)The expression of MMP-11 and-12 in cervical histological grade,clinical depth of stromal infiltration,with or without vascular invasion,with or without lymph stage,node metastasis was different.The difference was statistically significant ( P ＜0.05).(3)MMP-11 and-12 expression was significantly correlated.Conclusions The expression of MMP-11and-12 in cervical cancer tissues prompts that MMP-11 and-12 plays a central role in the course of invasion and metastasis of cervical carcinoma.MMP-11 and-12 joint detection may have a reference value in terms of the inchoate diagnosis of the cervical cancer,the evaluation and prognosis of tumor malignances degree.